Description: Cytochrome c oxidase [EC 1.9.3.1.] or Complex IV is the fourth complex of the electron Transport Chain located in the mitochondrial, and the principal terminal oxidase of high-affinity oxygen in the aerobic metabolism of all animals, plants, yeasts, and some bacteria. This enzyme is probably unique in providing energy for the cell by coupling electron transport through the cytochrome chain with the process of oxidative phosphorylation.The colorimetric assay in this kit is based on the observation of the decrease in absorbance at 550 nm of ferrocytochrome c caused by its oxidation to ferricytochrome c by cytochrome c oxidase. It is suitable for the detection of mitochondrial outer membrane integrity and for the detection of mitochondria in subcellular fractions. Storage ConditionsThe kit ships on wet ice and storage at –20°C is recommended. When stored unopened, the components in this kit are stable for 24 months. After the initial thawing of the 1 M Dithiothreitol Solution, divide the solution into undiluted working aliquots (still at 1 M concentration) and store at -20°C. Components and Storage This kit is sufficient for 100 tests. Storage at –20°C Assay Buffer 5 x 5 mL Enzyme Dilution Buffer 2x 2 mL Cytochrome c Solution 1 mL Dithiothreitol (DTT) Solution 30 µL n-Dodecyl β-D-maltoside Buffer 2 mL Cytochrom c Oxidase Positive Control 50 µL
Price: 546 USD
Location: Sunnyvale, California
End Time: 2025-01-14T23:17:01.000Z
Shipping Cost: 0 USD
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Item Specifics
All returns accepted: ReturnsNotAccepted
Brand: Tribioscience
Type: BIOCHEMICAL ASSAYS
MPN: TBS2115
Country/Region of Manufacture: United States
Intended Use/Discipline: Biological Laboratory, Medical Laboratory, Microbiological Laboratory, Physical Laboratory
