Description: Part I: OVERVIEW OF THE FIELD 1. A balance between autophagy and other cell death modalities in cancer Anna S. Gorbunova, Gelina S. Kopeina and Boris D. Zhivotovsky Part II: CHAPERONE-MEDIATED AUTOPHAGY (CMA) 2. Isolation of Autophagy competent Lysosomes from Cancer cells by Differential Large-scale Multi-layered Density Gradient Centrifugations Merve Kacal and Helin Vakifahmetoglu-Norberg. 3. CRISPR-Cas9 Gene Editing to create Isoform specific LAMP-2A Knockout in Human Cancer Cells Ting-ting Yu, Xun Zhou and Helin Vakifahmetoglu-Norberg Part III: MACROAUTOPHAGY 4. Modified LC3 dot quantification method Tahira Anwar and Eeva-Liisa Eskelinen. 5. A quantitative flow cytometry-based method for autophagy detection across the cell cycle Vitaly O. Kaminsky. 6. A dual HiBiT-GFP-LC3 lentiviral reporter for autophagy flux assessment Rainer Will, Katja Bauer, Matthias Kudla, Jetsy Montero-Vergara, Stefan Wiemann, Verena Jendrossek, Samuel Peña-Llopis and Silvia Vega-Rubin-de-Celis. 7. Measuring autophagic cargo flux with Keima-based probes Nikolai Engedal, Tonje Sønstevold, Carsten J. Beese, Sarvini Selladurai, Thea Melcher, Julia E. Simensen, Lisa B. Frankel, Alfonso Urbanucci and Maria L. Torgersen. 8. Detection of nuclear biomarkers for chromosomal instability Carles Pons, Eugenia Almacellas, Albert Tauler and Caroline Mauvezin. 9. Analysis of autophagic vesicles in mitotic cells Eugenia Almacellas, Marta Garcia-Cajide, Albert Tauler and Caroline Mauvezin. 10. Approaches and protocols to analyze autophagy and its role in death of apoptosis-resistant senescent tumor cells Elena Y. Kochetkova, Valery A. Pospelov and Tatiana V. Pospelova. 11. Concepts and design of introducing synthetic microRNAs into mammalian cells Per Hydbring. 12. Studying autophagy using a TMT-based quantitative proteomics approach Kiran Bala Sharma, Suruchi Aggarwal, Amit Kumar Yadav, Sudhanshu Vrati and Manjula Kalia. Part IV: MITOPHAGY13. Immunofluorescence-based measurement of autophagosome formation during mitophagy Benjamin S. Padman and Michael Lazarou. 14. Induction and detection of mitophagy Maria A. Yapryntseva, Boris Zhivotovsky and Vladimir Gogvadze. Part V: AUTOPHAGY ANALYSIS In vivo 15. Analysis of pancreatic acinar protein solubility in autophagy-deficient mice Matthew Smith, Carla Salomo-Coll, Morwenna Muir and Simon Wilkinson. 16. Studying autophagy in vivo in the mammary gland and in xenograft samples Zhongju Zou, Álvaro Fernández Fernández, Verena Jendrossek and Silvia Vega-Rubín-de-Celis. Part VI: COMPUTATIONAL APPROACHES 17. A method to Identify Potential Prognostic Markers Across Distinct Tumor Types Boxi Zhang, Elena Kochetkova and Erik Norberg. 18. A method for Co-Expression Analysis Boxi Zhang and Erik Norberg. Part VII: METABOLISM 19. Methodological approaches for assessing metabolomic changes in glioblastomas -MS Trang T. T. Nguyen, Enyuan Shang, Mike-Andrew Westhoff, Georg Karpel-Massler and Markus D. Siegelin 20. Extraction of Metabolites from Cancer Cells Elena Kochetkova, Tina Becirovic and Erik Norberg 21. Measuring glucose consumption and gluconeogenesis in 3D human tissue cultures with nanoliter input volumes Aurino M. Kemas and Volker M. Lauschke
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EAN: 9781071620731
UPC: 9781071620731
ISBN: 9781071620731
MPN: N/A
Format: Paperback, 353 pages, 2022 Edition
Author: Helin Norberg (Edited by)
Book Title: Autophagy and Cancer: Methods and Protocols (Metho
Item Height: 1.9 cm
Item Length: 25.4 cm
Item Weight: 0.64 kg
Item Width: 17.8 cm
Language: Eng
Publisher: Springer-Verlag New York Inc.
